FIFTH LAB REPORT : IMPATIENS BALSAMINA




ENVIRONMENTAL SOIL SCIENCE LAB FIVE REPORT
NUTRIENT ANALYSIS TEST

ENVIRONMENTAL SCIENCE (HS11)
SS11403 ENVIRONMENTAL SOIL SCIENCE
YEAR ONE SEMESTER 2 2017/2018

LECTURER: MDM. DIANA DEMIYAH
NAME
MATRIC NUMBER
CHAN CHU YIN
BS17110446
CHOW YIAN PENG
BS17110592
DARREN NETANIEL ERIC ROGERS
BS17110402
MIMORI SOGA
BS17270765
NUR AISYIKIN BINTI ABDULLAH
BS17160683
NUR DINIE DAYANA BINTI MOHAMAD RAFI
BS17110064



1.0 Introduction
Soil is the basis of almost everything we eat. Almost every food such as vegetables, fruits and grains are grown in soil. Meat production also depends on grazing or plant-based feeds from crops grown in soil. Soil nutrient test helps us to determine the nutrient concentration present in the soil sample. In this experiment, our group will analysis three nutrients which are Sulfate, Phosphorus and Nitrate by using HACH kit. HACH test kit provides a simple and effective way for analysis to determine nutrients content of the soil. Analysis of nutrients will undergo with the code of (680 Sulfate), (490 P React. PV-Phosphorus) and (355N, Nitrate HR PP). All the water sample will take 3 readings for each nutrient and average reading will be taken as final reading. Knowing present concentration of nutrients in soil informs us of a nutrient deficiency or surplus in soils used to support plant production.

2.0 Aims

To discover the suitable soil for the plant growth in terms of nutrients.

3.0 Objectives

1.    To obtain the skill of using HACH kit
2.    To analyze three major macronutrients (nitrogen, phosphorus, and potassium) for plants

4.0 Material and Apparatus:

20gram dried soil ( Black soil, red soil, sand ODEC , FSSA’s soil, Mangrove soil)
Distilled water
200mL glass beaker
Vacuum pump
High Density Polythene (HDPE) bottle
Square sample cell (10mL)

5.0 Procedure

1.       The soil sample must be dried at the outside of lab.
2.       20 gram of each soil samples were taken and poured into the beaker.
3.       50ml of distilled water was prepared and mixed with each of the soil samples by using spatula.
4.       After mixed well, the solution was let undisturbed at least 10 minutes.
5.       The 0.45µm membrane filter paper was placed on top of the vacuum pump.
6.       Vacuum pump switched on.
7.       After finished filter, the products were stored in the HDPE bottle and stored it in the refrigerator.
8.       The filtrated of each soil samples was poured into the three beakers.
9.       Then, three nutrients were analysed by using the HACH kit.
10.     Wear gloves all the time when conducting experiment by using HACH kit.
11.     The instruction followed in the manual book provided together with the HACH kit accordingly to each nutrients.
12.     Nutrient with code (680 Sulphate), (490 P React. PV- Phosphorus) and (355N, Nitrate HR PP) were analysed.
13.     3 readings for each nutrients and average of nutrients were taken.

6.0 Results

Type of soil
Nutrient
First reading (mg/L)
Second reading (mg/L)
Third reading (mg/L)
Average (mg/L)

         
Mangrove
680 Sulphate
82.00
82.00
91.00
85.00
490 Preact PV-phosphorus
2.50
2.50
2.50
2.50
355 N, Nitrate HR PP
0.62
0.62
0.62
0.62


ODEC
680 Sulphate
22.00
22.00
22.00
22.00
490 Preact PV-phosphorus
133.20
133.80
134.50
133.83
355 N, Nitrate HR PP
0.19
0.18
0.19
0.19


FSSA lake
680 Sulphate
29.00
29.00
28.00
28.67
490 Preact PV-phosphorus
15.40
14.80
14.90
15.03
355 N, Nitrate HR PP
0.42
0.41
0.40
0.41


Black soil
680 Sulphate
100
100
100
100
490 Preact PV-phosphorus
-0.70
-1.10
-1.10
-0.97
355 N, Nitrate HR PP
5.45
5.42
5.45
5.44


Tanah Bukit
680 Sulphate
5.00
4.00
5.00
4.67
490 Preact PV-phosphorus
9.90
9.90
9.80
9.87
355 N, Nitrate HR PP
0.39
0.39
0.39
0.39


7.0 Discussions

 Nutrient analysis purpose is to analyse the content of phosphate, nitrate and sulphate in the soil. This analysis test is conducted by using the HACH Test Kit which is 490 P react, PV - phosphorus, 355 Nitrate HR and 680 Sulphate. The reagent we use to test is collected by filtration because the medium in the saturated solution and not had many difference solution to do analysis. Nitrogen is important in plant growth where we can found in all plant cells. The source of soil nitrogen is atmospheric nitrogen. There are some plants for example legumes fix the atmospheric nitrogen in their roots and fertiliser from the factories use nitrogen to make ammonium sulphate and ammonium nitrate .

 Based on the result, the black soil sulphate content is 100 mg/L, the phosphorus is - 0.97 mg/L and the nitrate is 5.44 mg/L whereas for the Odec soil, the sulphate content 22.00 mg/L, phosphorus is 133.85 mg/L and nitrate is 0.19 mg/L. Mountain soil sulphate is 4.67 mg/L, phosphorus is 9.87 mg/L and nitrate is 0.39 mg/L while FSSA lake sulphate content 28.67 mg/L, phosphorus is 15.03 mg/L and nitrate is 0.41 mg/L . Lastly, the mangrove soil sulphate content  85.00 mg/L, phosphorus is 2.50 mg/L and nitrate 0.62 mg/L.

According to the result, the higher content of sulphate is black soil where its content is 100 mg/L while the lowest is mountain soil where the sulphate only contain 4.67 mg/L. The odec soil has high phosphorus which is 133.85 mg/L and black soil is -0.97 mg/L. Nutrient of nitrate is higher in Black soil than Odec soil is 0.19 mg/L. From the differents of each soil, the black soil has higher nutrient of sulphate and nitrate because of that the vegetation in black soil can growth easily. The odec soil the faster growth of vegetation because the higher of phosphorus but for long time it is became to die because lack of nutrient plant needed. The black soil the lowest nutrient of phosphorus.


8.0      Conclusion


          As a conclusion, yes, the knowledge and skill on how to use the HACH Kits are gained and from the result and observation, the three major nutrients which are nitrate, sulphate and phosphate are detected in the soil which are good for the plant growth. And from the observation, there is also factor that caused the nutrient’s content high and low such as fertilizer used, pollution and others.


Reference

HILLEL, D. (. (2005). Encyclopedia of soils in the environment. Retrieved May 9, 2018, from
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/soil-test

Multi-Parameter Kits. Retrieved May 9, 2018, from
https://www.hach.com/test-kits/multi-parameter-kits/family?productCategoryId=35547009712
Soil Nutrient Analysis: Nitrogen, Phosphorus, and Potassium. Retrieved May 7, 2018, from https://www.jove.com/science-education/10077/soil-nutrient-analysis-nitrogen-phosphorus-and-potassium




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